Beyoncé released the audio for her first single Bow Down/I Been On from her upcoming album on her Tumblr blog Sunday. The song is presumed to be the first track from her upcoming, as-yet-named album expected to be released at the end of the year.

"I took some time to live my life, but don't think I'm just his little wife," she sings. "Don't get it twisted, get it twisted, this my sh*t, bow down b---hes."

While some praised the song for the new direction from the mother-of-one, R&B singer Keyshia Cole called Beyoncé out indirectly- she didn't say her name- by suggesting she was a hypocrite for promoting unity in previous songs and to suggest that people "bow down b*tches."

"Can't stand when people all self-righteous when it's convenient it makes them look good. Lmao! But can still talk sh*t when convenient 2 FOH," Cole tweeted.

"I done kept it real from the start! #RespectTHAT," Cole continued.

"First 'Women need to Stick together' now b*tches better Bow. Smh. But it's all G! Chicks stay shooting the sh*t. But when I speak my mind its a prob. #Well #StayMad.," she added.

A Beyoncé fan defended the Halo singer in a tweet: "f*ck yo unity."

"Never said there was any. IM not in denial... God Bless," Cole replied.

Another Beyonce fan told Cole she was "hating on everybody," and the singer replied: "How am I hating? no Mamm or sir. whatever u may be. No H8."

Cole then plugged her appearance on Monday's Jimmy Kimmel Live.

This is not the first time there's been beef between Cole and Beyoncé's fan base.

When Kelly Rowland and Michelle Williams joined Beyoncé onstage for her 2013 Super Bowl halftime performance on Feb. 3, Cole criticized Williams' vocals on Twitter.

"I think I was frightened to blink for a sec. Then Michell sung and woke my ass up from my daze!" Cole reportedly wrote on Twitter. "She always f*ckN the groove up."

After Destiny's Child fans gave Cole backlash on the social media site, she fired back: "But hey! I love y'all too! And y'all girl is WACK and always will be."

Rowland, 32, addressed Cole's comments in a recent interview.

"It didn't even make it on my radar until other people brought it up," Rowland told Rolling Stone. "It didn't even matter. We had a wonderful performance, and that's all I cared about. I was with my sisters and the three of us matter to each other, and that's all that counts," she said of the reunion. "We matter to a lot more people, with all due respect, than her. So, I really don't care what she said."