Photos from Beyonce's recent concert in Puerto Rico caused speculation that she is with pregnant again.

Media Takeout published photos on Monday of the Single Ladies singer taken at her The Mrs Carter Show concert at the Coliceo de Puerto Rico venue in San Juan, Puerto Rico on Saturday.

In one picture, the side view angle of the songstress wearing a green leopard print dress makes her appear to have a growing baby bump.

According to the report, sources revealed the mother-of-one has already stopped her tradition of drinking champagne at the end of every concert, possibly because she is in the early stages of her next pregnancy.

View the alleged baby bump photos here.

Beyonce Pregnant Again? Baby Bump in London & Met Gala 2013 Dress Spark Rumors (PHOTO, VIDEO) 

Several incidents sparked rumors that hip-hop couple Beyonce and Jay-Z are expecting a second child. When she wore a blue sparkle catsuit and called her daughter Blue Ivy to the stage at London's O2 Arena in May, a fan photo taken of the singer from the side made her appear to have a baby bump.

On May 6, the multi-Grammy winner attended Metropolitan Museum of Art's Gala for the exhibit Punk: Chaos Couture, wearing a black strapless high-waisted Givenchy Couture gown. Beyoncé told The Huffington Post the corset to her dress was so tight that she was going to "need scissors to cut the string."

A week later on May 13, New York Post published a report that "multiple" entertainment industry insiders who also attended Metropolitan Museum of Art's Gala said she wore the corseted high-waisted dress because it "camouflaged" her growing baby bump.

Beyonce was interviewed by TODAY co-anchor Savannah Guthrie while backstage at Chime For Change: The Sound of Change Live Concert on June 1. She was sitting alongside Salma Hayek and Gucci's Creative director Frida Giannini.

Guthrie told the mother-of-one, "I would be remiss if I didn't give you a chance to dispel any rumors about whether you're expecting."

The singer didn't confirm or deny in her answer: "What kind of rumor? Huh?" Beyoncé asked, and added, "I don't know what you're talking about." She then burst into laughter with Hayek and Giannini.

Beyonce Laughs Off Pregnancy Rumors on 'TODAY' During 'Chime For Change' Concert Interview (VIDEO) 

Jay-Z later denied that Beyoncé is pregnant with the couple's second child.

The hip-hop mogul debunked the rumor in a reply email to New York radio station host Ebro Darden of Hot 97 FM, who sent an email congratulating him on the news.

Blue Ivy, Beyonce Birthday Yacht Vacation PHOTOS With Jay Z; Family Celebrate 'Grown Woman' Singer's Birthday (PICS) 

Watch the shocking video where Beyonce was almost pulled off of a stage in a Brazil concert earlier this month below